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KC-4M1 [KC-4(IgM)]
KC-4M1 [KC-4(IgM)]
規(guī)格:
價格:詢價
編號:B164906
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
KC-4M1 [KC-4(IgM)]
商品貨號
B164906
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Adenocarcinoma
Applications
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues.
An antibody with the same specificity but of the IgG3 subclass is available (ATCC HB-8709).
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human carcinoma cells
Cellular Products
immunoglobulin; monoclonal antibody; against human carcinoma cells
Comments
Animals were immunized with prostatic adenocarcinoma cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues.
The antigen is found in the cytoplasm as a 490000 dalton molecule, and in the membrane and cytoplasm as a 438000 dalton molecule.
An antibody with the same specificity but of the IgG3 subclass is available (ATCC HB-8709).
Complete Growth Medium
Culture medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.015
mM thymidine, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype
IgM
Name of Depositor
Coulter Corporation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987

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