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L203
L203
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B164938
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
L203
商品貨號(hào)
B164938
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody has the same specificity as L243 (ATCC HB-55) but is not cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3/NSI-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human Ia
Cellular Products
immunoglobulin; monoclonal antibody; against human Ia
Comments
Animals were immunized with RPMI 8866 human lymphoblastoid cells.
Spleen cells were fused with P3/NSI-Ag4-1 myeloma cells.
The antibody has the same specificity as L243 (ATCC HB-55) but is not cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
LA Lampson
Deposited As
mouse (B cell); mouse (myeloma)
References

Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line. J. Immunol. 125: 293-299, 1980. PubMed: 6966655

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