Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender
female
Applications
The cells are resistant to methotrexate.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
female
Comments
The cells are resistant to methotrexate.
Complete Growth Medium
Alpha minimum essential medium with 0.001 mM methotrexate, 90%; dialyzed heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks.
