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LBRM-33 clone 4A2
LBRM-33 clone 4A2
規格:
價格:詢價
編號:B164963
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
LBRM-33 clone 4A2
商品貨號
B164963
Organism
Mus musculus, mouse
Cell Type
T lymphocyte; radiation induce
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension; some adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
B10.BR
Applications
This line was derived from a radiation induced T cell lymphoma.
Production of IL-2 is stimulated by T cell mitogens.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from a radiation induced T cell lymphoma.
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Cellular Products
interleukin-2 (interleukin 2, IL-2)
Comments
This line was derived from a radiation induced T cell lymphoma.
Production of IL-2 is stimulated by T cell mitogens.
Peak levels are found in the culture medium after 24 hours of stimulation with either 1% PHA or 20 microgm/ml Con A.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml Adherent cells can be dislodged by scraping with subsequent resuspension as above.
Cryopreservation
90% FBS + 10% DMSO
Name of Depositor
P Ralph
Deposited As
Mus musculus
References

Gillis S, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of interleukin-2 producing T cell lymphomas. J. Immunol. 125: 2570-2578, 1980. PubMed: 6968790

Mochizuki D, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma. J. Immunol. 125: 2579-2583, 1980. PubMed: 7000903

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