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L-M
L-M
規格:
價格:詢價
編號:B164989
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
L-M
商品貨號
B164989
Organism
Mus musculus, mouse
Tissue
subcutaneous connective tissue; areolar and adipose
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
100 days
Gender
male
Strain
C3H/An
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
male
Antigen Expression
H-2k
Virus Resistance
poliovirus 1, 2, 3
Comments
Grows in protein free medium.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Medium 199 with Earle's BSS supplemented with 0.5% Bacto-peptone
Subculturing
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor
DJ Merchant
Deposited As
Mus musculus
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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