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LMH
LMH
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164992
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
LMH
商品貨號(hào)
B164992
Organism
Gallus gallus, chicken
Tissue
liver
培養(yǎng)基
Cell Type
chemically induced
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hepatocellular carcinoma
Strain
Leghorn
Applications
transfection host
Storage Conditions
liquid nitrogen vapor phase
Karyotype
triploid; modal number = 116; six marker chromosomes
Derivation
LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.
Clinical Data
Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
Receptor Expression
estrogen receptor, positive
Tumorigenic
Yes
Effects
Yes, forms tumors in nude mice
Comments
Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
The morphology of the cells is dendritic like.
The cells express glucose-6-phosphatase and weak canalicular ATPase activity.
The cells form tumors in athymic nude mice.
LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene.
The cell line is useful for transfection studies.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.
Complete Growth Medium
Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution.
Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate, and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.


Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
T Kitagawa, DL Williams
Deposited As
Gallus gallus
Year of Origin
1981
References

Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000

Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775

Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707

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