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LYK-16
LYK-16
規格:
貨期:
編號:B165020
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
LYK-16
商品貨號
B165020
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: S344NCR (B cell); BALB/c (myeloma)

Applications
Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.
The antibody is specific for a determinant that is apparently dependent upon posttranslational modification of CD44.
The specific exon coding for the determinant is not known.
Derivation
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a mouse CD44 determinant dependent upon posttranslational modification (specific exon unknown)
Cellular Products
immunoglobulin; monoclonal antibody; against a mouse CD44 determinant dependent upon posttranslational modification (specific exon unknown)
Comments
Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody is specific for a determinant that is apparently dependent upon posttranslational modification of CD44.
The specific exon coding for the determinant is not known.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with: 0.05 mM 2-mercaptoethanol 1% Origen Hybridoma Cloning Factor 10% fetal bovine serum
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
KS Hathcock, Y Yamashita
Deposited As
rat (B cell); mouse (myeloma)
References

Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314

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