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Lym-2
Lym-2
規(guī)格:
價格:詢價
編號:B165026
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Lym-2
商品貨號
B165026
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Genes Expressed
immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells
Cellular Products
immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells
Comments
Animals were immunized with CLL cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Northwestern University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Epstein AL. Murine hybridoma Lym-2 and diagnostic antibody produced thereby. US Patent 4,724,212 dated Feb 9 1988

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