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M059K
M059K
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165031
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
M059K
商品貨號(hào)
B165031
Organism
Homo sapiens, human
Tissue
brain
培養(yǎng)基
Cell Type
glial cell
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
malignant glioblastoma; glioma
Age
33 years
Gender
male
Applications
These cell lines provide useful model systems in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Images
Derivation
M059K cells were isolated from a tumor specimen from a 33 year-old male patient with untreated glioblastoma. These cells were isolated concurrently from the same tumor specimen as M059J (see ATCC CRL-2366). -
Clinical Data
male
33 years
Tumorigenic
Yes
Effects
Yes, forms tumors in SCID mice
Comments
M059K cells express normal levels of DNA-dependent protein kinase while M059J cells lack DNA-dependent protein kinase activity. M059K cells are approximately 30-fold less sensitive to ionizing radiation than M059J cells. M059K cells are less sensitive than M059J cells to the cytotoxic effects of bleomycin, N, N-bis(2-chloroethyl)-N-nitrosourea and nitrogen mustard. M059K cells are DNA double strand break repair proficient. Both cell lines are negative for glial fibrillary acidic acid (GFAP). Together these cell lines provide useful model systems in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair.
Complete Growth Medium
These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:6 to 1:8
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 14
D16S539: 10,12
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8
vWA: 17
Name of Depositor
J Allalunis-Turner, RS Day
Deposited As
human
References

Allalunis-Turner MJ, et al. Isolation of two lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs. Radiat. Res. 134: 349-354, 1993. PubMed: 8316628

Lees-Miller SP, et al. Absence of p350 subunit of DNA activated protein kinase from a radiosensitive human cell line. Science 267: 1183-1185, 1995. PubMed: 7855602

Allalunis-Turner J, et al. Intact G2-phase checkpoint in cells of a human cell line lacking DNA-dependent protein kinase activity. Radiat. Res. 147: 284-287, 1997. PubMed: 9052673

Allalunis-Turner MJ, et al. Radiation-induced DNA damage and repair in cells of a radiosensitive human malignant glioma cell line. Radiat. Res. 144: 288-293, 1995. PubMed: 7494872

Wang J, et al. Radiation-induced damage in two human glioma cell lines as measured by the nucleoid assay. Anticancer Res. 17: 4615-4618, 1997. PubMed: 9494578

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