91麻豆成人精品国产,中文日韩欧美,xx久久

MA-104 Clone 1

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B165058

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱

MA-104 Clone 1

商品貨號(hào)

B165058

Organism

Cercopithecus aethiops

Tissue

kidney

培養(yǎng)基

Cell Type

epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

embryo

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Derivation

An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue.

Virus Susceptibility

Simian rotavirus

Comments

This item is not available for international distribution.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 5 x 10³ to 7 x 10³ viable cells/cm² is recommended.
Incubate cultures at 37°C

Subculture Ratio: 1:3 to 1:10

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Population Doubling Time

27 hours

Name of Depositor

MM Vincent

Year of Origin

2001

References

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530

MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1).

王經(jīng)理

手機(jī)號(hào)：17280875617

QQ：1438578920
胡經(jīng)理

手機(jī)號(hào)：13345964880

QQ：2438244627
周經(jīng)理

手機(jī)號(hào)：17757487661

QQ：1296385441
于經(jīng)理

手機(jī)號(hào)：18067160830

QQ：2088210172
沈經(jīng)理

手機(jī)號(hào)：19548299266

QQ：2662369050
李經(jīng)理

手機(jī)號(hào)：13626845108

QQ：972239479
曹經(jīng)理

手機(jī)號(hào)：18662422060

QQ：3671869992