Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Dulbecco's Modified Eagle's Medium, 4.5 g/L glucose with 0.1 mM non-essential amino acids, 90%; fetal bovine serum, 10%
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/ml and maintain between 1 x 105 and 1 x 106 cells/ml.
Medium Renewal: Every 2 to 3 days
Tzartos SJ, et al. Mapping of surface structures of Electrophorus acetylcholine receptor using monoclonal antibodies. J. Biol. Chem. 256: 8635-8645, 1981. PubMed: 6167581
Tzartos SJ, et al. Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 79: 188-192, 1982. PubMed: 6948300
Sargent PB, et al. Structure and transmembrane nature of the acetylcholine receptor in amphibian skeletal muscle as revealed by cross-reacting monoclonal antibodies. J. Cell Biol. 98: 609-618, 1984. PubMed: 6363425
Swanson LW, et al. Immunohistochemical localization of monoclonal antibodies to the nicotinic acetylcholine receptor in chick midbrain. Proc. Natl. Acad. Sci. USA 80: 4532-4536, 1983. PubMed: 6192437
