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當(dāng)前位置: 首頁 > ATCC代理 > MC/9 B165096
MC/9
MC/9
規(guī)格:
價格:詢價
編號:B165096
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
MC/9
商品貨號
B165096
Organism
Mus musculus, mouse
Tissue
liver
培養(yǎng)基
MCM2268：MC/9細(xì)胞專用培養(yǎng)基
Cell Type
mast cell
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
13 days
Strain
(B6 X A/J)F1
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This is a cloned mast cell line derived from the fetal liver of a (B6 X A/J)F1 mouse.
Receptor Expression
FcERI (Fc of IgE)
Genes Expressed
histamine; leukotrienes
Cellular Products
histamine; leukotrienes
Comments
The cells can be sensitized to specific antigens by incubating them with IgE having the desired antigenic specificity.

The cells so coated will release soluble mediators (such as histamine) in response to exposure to the antigen (allergen).


Complete Growth Medium
Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10% Rat T-STIM (Becton Dickenson Catalog No. 354115) and 10% fetal bovine serum.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 105 viable cells/ml. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL. Do not allow the cell density to exceed 2 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor
Dana Farber Cancer Inst.
Deposited As
Mus musculus
U.S. Patent Number
References

Cantor HI, Nabel G. Assay methods and systems utilizing mast cell clones. US Patent 4,559,310 dated Dec 17 1985

Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154

Galli SJ, et al. Mast cell clones: a model for the analysis of cellular maturation. J. Cell Biol. 95: 435-444, 1982. PubMed: 6216259

Lewin I, et al. Aggregation of the FcepisilonRI in mast cells induces the synthesis of fos-interacting protein and increases its DNA binding activity: the dependence on protein kinase c-beta. J. Biol. Chem. 271: 1514-1519, 1996. PubMed: 8576146

Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

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