黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > MCB3901 [AV12-664] B165106
MCB3901 [AV12-664]
MCB3901 [AV12-664]
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165106
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
MCB3901 [AV12-664]
商品貨號(hào)
B165106
Organism
Mesocricetus auratus, hamster, Syrian golden
Cell Type
adenovirus 12 induced tumor
培養(yǎng)基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2  [Cells contain Adenovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This cell line can be used as a transfection host for exogenous gene expression using the BK virus enhancer - adenovirus late promoter system.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype
modal chromosome number is approximately 46
Genes Expressed
early region proteins of adenovirus 12
Cellular Products
early region proteins of adenovirus 12
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:8 to 1:20
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Culture Conditions
Temperature: 37°C
Population Doubling Time
14 to 16 hrs
Name of Depositor
Eli Lilly & Co.
Deposited As
Mesocricetus auratus
U.S. Patent Number
References

Beavers LS, et al. Recombinant and chimeric KS1/4 antibodies directed against a human adenocarcinoma antigen. US Patent 4,975,369 dated Dec 4 1990

Berg DT, et al. E1a-responsive mammalian host/vector system for the stable high-level expression of secreted proteins. Nucleic Acids Res. 20: 5485-5486, 1992. PubMed: 1437573

Grinnell BW, et al. Gamma-carboxylated isoforms of recombinant human protein S with different biologic properties. Blood 76: 2546-2554, 1990. PubMed: 2148275

Burck PJ, et al. Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain. J. Biol. Chem. 265: 5170-5177, 1990. PubMed: 2108167

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

  • 王經(jīng)理
    手機(jī)號(hào)：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號(hào)：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號(hào)：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號(hào)：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號(hào)：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號(hào)：13626845108
    QQ：972239479
  • 曹經(jīng)理
    手機(jī)號(hào)：18662422060
    QQ：3671869992