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MLE 12
MLE 12
規格:
價格:詢價
編號:B165163
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MLE 12
商品貨號
B165163
Organism
Mus musculus, transgenic, mouse, transgenic
Tissue
lung
培養基
Cell Type
epithelial; SV40 transformed
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2    [Cells contain Papovavirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
5 month old
Gender
female
Strain
FVB/N
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene.
Clinical Data
female
5 months
Genes Expressed
lung surfactant proteins B and C (SP-B, SP-C)
Tumorigenic
Yes
Effects
Yes, tumors were generated within 6 to 9 months in nude mice
Comments
The cells express the mRNA for large T antigen.
Lung surfactant proteins B and C were detected.
The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin.
Complete Growth Medium
HITES medium supplemented with 2% fetal bovine serum
    HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows:
  • Dulbecco's medium : Ham's F12, 50:50 mix (ATCC® 30-2006)
  • Insulin 0.005 mg/ml
  • Transferrin 0.01 mg/ml
  • Sodium selenite 30 nM
  • Hydrocortisone 10 nM
  • β-estradiol 10 nM
  • HEPES 10 mM
  • L-glutamine (ATCC® 30-2214) 2 mM (in addition to that in the base medium)
  • Fetal bovine serum (ATCC®  30-2020) 2% 

Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time
7 to 9 hrs
Name of Depositor
JA Whitsett
Deposited As
mouse, transgenic
Year of Origin
1992
References

Wikenheiser KA, et al. Simian virus 40 large T antigen directed by transcriptional elements of the human surfactant protein C gene produces pulmonary adenocarcinomas in transgenic mice. Cancer Res. 52: 5342-5352, 1992. PubMed: 1394139

Wikenheiser KA, et al. Production of immortalized distal respiratory epithelial cell lines from surfactant protein C/simian virus 40 large tumor antigen transgenic mice. Proc. Natl. Acad. Sci. USA 90: 11029-11033, 1993. PubMed: 8248207

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