黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Mo [Mo T] B165181
Mo [Mo T]
Mo [Mo T]
規格:
價格:詢價
編號:B165181
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Mo [Mo T]
商品貨號
B165181
Organism
Homo sapiens, human
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2  [Cells contain Retrovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hairy cell leukemia
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Genes Expressed
granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon
Cellular Products
granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon
Comments

A plasmid containing the DNA sequence coding from Mo that codes for CSF is available as ATCC 39754.

Mo cells contain a replication competent genome of Human T Cell Leukemia Virus II (HTLV-II) and two defective HTLV-II genomes.

For long term culture, the cells can be grown in medium with 2% fetal bovine serum.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
The cells are subcultured every 7 days by centrifugation and division of the cell pellet 1:4 (1:6 maximum). Do not dilute below 2 X 105 viable cells/ml or allow to exceed 2 X 106 cells/ml. Culture at 5% CO2.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6
Medium Renewal: Add medium every 2 to 3 days depending on cell density

Note:The donor suggests using Falcon flasks for optimum growth.

Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 8 to 9% CO2 in air
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,13
D16S539: 11
D5S818: 11,12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 17,18
Name of Depositor
Genetics Institute, Inc., Regents of the University of California
U.S. Patent Number
References

Golde DW, Quan SG. Unique T-lymphocyte line and products derived therefrom. US Patent 4,438,032 dated Mar 20 1984

Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987

Saxon A, et al. Immunologic characterization of hairy cell leukemias in continuous culture. J. Immunol. 120: 777-782, 1978. PubMed: 344797

Gasson JC, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 226: 1339-1342, 1984. PubMed: 6390681

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479
  • 曹經理
    手機號:18662422060
    QQ:3671869992