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MRSS-1 (D2D4)
MRSS-1 (D2D4)
規格:
價格:詢價
編號:B165195
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MRSS-1 (D2D4)
商品貨號
B165195
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells.
The antibody cross-reacts with double stranded DNA and polyinosinic-cytidylic acid (poly I-C).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells.
Clinical Data
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against DNA (single stranded)
Cellular Products
immunoglobulin; monoclonal antibody; against DNA (single stranded)
Comments
Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells.
The antibody cross-reacts with double stranded DNA and polyinosinic-cytidylic acid (poly I-C).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG3
Name of Depositor
BH Hahn, F Ebling
Deposited As
mouse (B cell); mouse (myeloma)
References

Hahn BH, et al. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 23: 942-945, 1980. PubMed: 6157395

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