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MYA-1

規格:

價格:詢價

編號:B165209

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MYA-1

商品貨號

B165209

Organism

Felis catus, cat

Tissue

peripheral blood

培養基

Cell Type

T lymphoblast

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

pathogen-free

Age

5 months

Storage Conditions

liquid nitrogen vapor phase

Derivation

MYA-1 is a T lymphoblastoid cell line that was established from blood mononuclear cells from a specific pathogen-free cat in 1988.

Antigen Expression

feline CD3 +; feline CD4 +; feline CD8 -

Receptor Expression

interleukin 2 (IL-2)

Comments

The cells are interleukin-2 (Il-2) dependent. They are positive for IL-2 receptor, feline CD3, feline CD4 and major histocompatibility complex (MHC) class II antigen. They are negative for feline CD8.

FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells. RefMiyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 units/ml recombinant human IL-2 and 10% fetal bovine serum

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10⁵ viable cells/mL. Maintain cell density between 1 x 10⁵and 1 x 10⁶ viable cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Name of Depositor

T Miyazawa

Deposited As

cat

Year of Origin

1988

References

Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760

Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703

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