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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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N1.551
N1.551
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165216
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
N1.551
商品貨號(hào)
B165216
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the fast IIa isoform of rodent skeletal muscle myosin.
It cross-reacts with the IIx isoform, and does not react with the IIb isoform.
Derivation
Animals were immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rodent fast IIa myosin heavy chain (MyHC)
Cellular Products
immunoglobulin; monoclonal antibody; against rodent fast IIa myosin heavy chain (MyHC)
Comments
Animals were immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with the fast IIa isoform of rodent skeletal muscle myosin.
It cross-reacts with the IIx isoform, and does not react with the IIb isoform.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml 10 to 15 minutes, the flask may need to be shaken firmly to dislodge the cells).
Add fresh medium, aspirate and dispense into new flasks.
Isotype
IgM
Name of Depositor
HM Blau
Deposited As
mouse (B cell); mouse (myeloma)
References

Condon K, et al. Development of muscle fiber types in prenatal rat hindlimb. Dev. Biol. 138: 256-274, 1990. PubMed: 2108065

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