黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > NCCIT B165228
NCCIT
NCCIT
規格:
價格:詢價
編號:B165228
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCCIT
商品貨號
B165228
Organism
Homo sapiens, human
Tissue
anterior mediastinal mixed germ cell tumor
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
pluripotent embryonal carcinoma; teratocarcinoma
Age
adult
Gender
male
Ethnicity
Japanese
Images
Derivation
NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor.
Clinical Data
adult
Japanese
male
Tumorigenic
Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
This pluripotent stem cell line is capable of somatic and extraembryonic differentiation.
The undifferentiated cells are equivalent to a stage intermediate between seminoma and embryonal carcinoma.
They will differentiate in response to retinoic acid.
NCCIT cells are negative for keratin.
They are positive for vimentin and placental alkaline phosphatase.
The line was contaminated with mycoplasma, but was cured by the depositor prior to deposit at the ATCC.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture two times weekly.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 10,13
D7S820: 10
THO1: 7,9
TPOX: 8
vWA: 14,18
Name of Depositor
I Damjanov
Deposited As
Homo sapiens
Year of Origin
1985
References

Damjanov I, et al. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab. Invest. 68: 220-232, 1993. PubMed: 7680083

Teshima S, et al. Four new human germ cell tumor cell lines. Lab. Invest. 59: 328-336, 1988. PubMed: 2842544

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479
  • 曹經理
    手機號：18662422060
    QQ：3671869992