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NCI-BL1770 [BL1770]

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價格:詢價

編號:B165236

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

NCI-BL1770 [BL1770]

商品貨號

B165236

Organism

Homo sapiens, human

Tissue

peripheral blood

培養基

Cell Type

B lymphoblast; Epstein-Barr virus (EBV) transformed

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

2 [Cells contain Epstein-Barr Virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

57 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Derivation

The carcinoma cell line established from the same patient is available as CRL-5893.

Clinical Data

57 years

Caucasian

male

Complete Growth Medium

ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite (final conc.)
50 nM Hydrocortisone (final conc.)
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine (final conc.)
0.01 mM phosphorylethanolamine (final conc.)
100 pM triiodothyronine (final conc.)
0.5% (w/v) bovine serum albumin (final conc.)
10 mM HEPES
0.5 mM sodium pyruvate (final conc.)
extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁶ viable cells/mL. Maintain cell density between 1 x 10⁶ and 3 x 10⁶ cells/mL.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).

Cryopreservation

Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Name of Depositor

AF Gazdar, JD Minna

Deposited As

Homo sapiens

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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