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當(dāng)前位置: 首頁 > ATCC代理 > NCI-H1341 [H1341] B165257
NCI-H1341 [H1341]
NCI-H1341 [H1341]
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165257
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H1341 [H1341]
商品貨號(hào)
B165257
Organism
Homo sapiens, human
Tissue
lung; Derived from metastatic site: cervix
培養(yǎng)基
Product Format
frozen
Morphology
floating aggregates of round cells with a few attached cells.
Culture Properties
suspension
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma; small cell lung cancer
Gender
female
Applications
The line was established in February 1986.
Derivation
The line was established in February 1986.
Clinical Data
The tissue donor was a non-smoker.
female
Comments
The line was established in February 1986.
The tissue donor was a non-smoker.
Complete Growth Medium
ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium.
Cells grow as floating aggregates of round cells with a few attached cells.
Cryopreservation
Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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