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當(dāng)前位置: 首頁 > ATCC代理 > NCI-H1355 [H1355] B165258
NCI-H1355 [H1355]
NCI-H1355 [H1355]
規(guī)格:
價(jià)格:詢價(jià)
編號:B165258
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H1355 [H1355]
商品貨號
B165258
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: pleural effusion
培養(yǎng)基
Product Format
frozen
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage 4, adenocarcinoma
Age
53 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 54; range = 43-66
Derivation
The cell line was established in March 1986 from pleural effusion metastasis of lung.
Clinical Data
53 years
Caucasian
male
Complete Growth Medium
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing
Cultures can be maintained by the addition of fresh medium or by replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension of the pellet. The cells grow in floating aggregates of round cell clusters.
Medium Renewal: twice weekly
Note: Add fresh medium as the cell density increases.
Cryopreservation
Freeze medium: Complete culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 11,13
D7S820: 12
THO1: 9
TPOX: 9,12
vWA: 14,17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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