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當前位置: 首頁 > ATCC代理 > NCI-H1436 [H1436] B165265
NCI-H1436 [H1436]
NCI-H1436 [H1436]
規格:
價格:詢價
編號:B165265
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H1436 [H1436]
商品貨號
B165265
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: lymph node
培養基
Product Format
frozen
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, carcinoma; classic small cell lung cancer
Age
39 years adult
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
3p is normal
Clinical Data
3 years old
male
Caucasian
The tumor developed in a patient with a 75 packs-year history of cigarette smoking.

Comments

The tumor developed in a patient with a 75 packs-year history of cigarette smoking.

Complete Growth Medium
The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the base medium: 
  • 0.005 mg/ml Insulin (Sigma I1882)
  • 0.01 mg/ml Transferrin (Sigma T1147)
  • 30nM Sodium selenite (final conc.) (Sigma S9133) 
  • 10 nM Hydrocortisone (final conc.) (Sigma H0135)
  • 10 nM beta-estradiol (final conc.) (Sigma E2557)  
  • extra 2mM L-glutamine (for final conc. of 4.5 mM) (Sigma G5783) 
  • 5% fetal bovine serum (ATCC 30-2020)
Subculturing
Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2 in air 
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 11,13
D7S820: 9,11
THO1: 7,9.3
TPOX: 9,11
vWA: 17,18
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
June, 1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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