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NCI-H220 [H220]
NCI-H220 [H220]
規格:
價格:詢價
編號:B165325
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H220 [H220]
商品貨號
B165325
Organism
Homo sapiens, human
Tissue
lung
培養基
Product Format
frozen
Culture Properties
suspension, floating aggregates of round cells with some attached cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E,carcinoma; classic small cell lung cancer
Age
51 years
Gender
male
Ethnicity
Black
Applications
The line was established in February 1980.
Derivation
The line was established in February 1980.
Clinical Data
The tissue donor was a non-smoker.
male
Black
51 years
Comments
The line was established in February 1980.
The tissue donor was a non-smoker.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 3 to 5 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 12
D16S539: 10
D5S818: 11
D7S820: 10, 11
TH01: 7,9.3
TPOX: 8,11
vWA: 16,18
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1980
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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