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NCI-H2347 [H2347]
NCI-H2347 [H2347]
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165334
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H2347 [H2347]
商品貨號(hào)
B165334
Organism
Homo sapiens, human
Tissue
lung
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage 1, adenocarcinoma; non-small cell lung cancer
Age
54 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established in May 1990 from lung adenocarcinoma.

Clinical Data
54 years
A lymphoblastoid line from the same patient is available as ATCC CRL-5970.
Caucasian
female
The tissue donor was a non-smoker.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
    Medium Renewal: Every 2-3 days
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 11
    D13S317: 12,14
    D16S539: 11
    D5S818: 11
    D7S820: 10,11
    THO1: 9.3
    TPOX: 8
    vWA: 16,19
    Name of Depositor
    AF Gazdar, JD Minna
    Deposited As
    Homo sapiens
    Year of Origin
    1990
    References

    NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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