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NCI-H250 [H250]
NCI-H250 [H250]
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B165338
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
NCI-H250 [H250]
商品貨號(hào)
B165338
Organism
Homo sapiens, human
Tissue
lung
培養(yǎng)基
Product Format
frozen
Culture Properties
aggregates in suspension; the cells grow in very large aggregates, and the aggregates are the only viable cell population
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, carcinoma; classic small cell lung cancer
Age
34 years
Gender
male
Ethnicity
Black
Storage Conditions
liquid nitrogen vapor phase
Karyotype
3p is normal
Images
Cell Micrograph of ATCC CRL-5828, NCI-H250
Derivation
The line was derived by A. F. Gazdar, H. K. Oie, J. D. Minna and associates from a tumor that developed in a non-smoker.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 13
D13S317: 12
D16S539: 9,13
D5S818: 11
D7S820: 8,11
THO1: 9
TPOX: 8,11
vWA: 15,16
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
June, 1980
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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