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NFS-25 C-3
NFS-25 C-3
規(guī)格:
價(jià)格:詢價(jià)
編號:B165392
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NFS-25 C-3
商品貨號
B165392
Organism
Mus musculus, mouse
Cell Type
pre-B lymphoblast
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
NFS.C58v-1
Applications
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
Antigen Expression
CD5 +; CD32 +; CD45R +
Genes Expressed
CD5 +; CD32 +; CD45R +
Comments
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
The cells bear markers consistent with identification as large pre-B lymphoblasts [(Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B220), CD45R)].
They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 10 mM HEPES, 3 mM glutamine, 1 mM oxaloacetic acid, 0.2 units/ml insulin, 0.5 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 2 mcg/ml transferrin, 80%; NCTC medium 109, 10%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor
WJ Davidson, HC Morse
Deposited As
Mus musculus
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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