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NMuMG
NMuMG
規格:
價格:詢價
編號:B165404
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NMuMG
商品貨號
B165404
Organism
Mus musculus, mouse
Tissue
mammary gland
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender
female
Strain
NAMRU
Applications
This cell line is a suitable transfection host
Clinical Data
female
Tumorigenic
Yes
Effects
Yes, benign cystadenomas are produced in isogenic mice
Comments
The cells do not express detectable retroviruses.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Name of Depositor
RB Owens
Deposited As
Mus musculus
References

Owens RB. Glandular epithelial cells from mice: a method for selective cultivation. J. Natl. Cancer Inst. 52: 1375-1378, 1974. PubMed: 4363715

Owens RB, et al. Epithelial cell cultures from normal glandular tissue of mice. J. Natl. Cancer Inst. 53: 261-269, 1974. PubMed: 4366196

Yingling JM, et al. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc. Natl. Acad. Sci. USA 93: 8940-8944, 1996. PubMed: 8799132

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