Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
These cells are grown in Dulbecco?s Modified Eagle?s Medium/Ham?s Nutrient Mixture F-12, 1:1 with 2.5 mM L-glutamine, 1.2 g/L sodium bicarbonate, 15mM HEPES and 0.5mM sodium pyruvate.
Supplemented with:
This medium is formulated for use with a 5% CO2 in air atmosphere.
ATCC modified and tested medium formulation (without the additional supplements or serum described above) is available as ATCC 30-2006.
ATCC tested fetal bovine serum is available as ATCC 30-2020 (500ml) and ATCC 30-2021 (100ml).
