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OKT 3
OKT 3
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165432
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
OKT 3
商品貨號(hào)
B165432
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
Spleen
培養(yǎng)基
Cell Type
Hybridoma: B lymphocyte
Product Format
frozen
Morphology
Lymphoblast
Culture Properties
Suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
CAF1 (B cell); BALB/c (myeloma)
Shipping Information
Frozen
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Mice were immunized with human peripheral blood lymphocytes. Spleen cells were fused with P3x63Ag8.U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte); against CD3
Cellular Products
immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte); against CD3
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Complete culture medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Isotype
IgG2a
Name of Depositor
Ortho Pharmaceutical Corporation
U.S. Patent Number
References

Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909

Kung PC, Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. US Patent 4,361,549 dated Nov 30 1982

Tanaka Y, et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 8985372

Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218

Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792

Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998

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