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PA317 containing JR-gal
PA317 containing JR-gal
規格:
價格:詢價
編號:B165467
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PA317 containing JR-gal
商品貨號
B165467
Organism
Mus musculus, mouse
Tissue
embryo
培養基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
embryo
Strain
NIH/Swiss
Applications
This cell line was derived from PA317 (see ATCC CRL-9098) by introduction of a retrovirus vector (JR-gal).
The vector is derived from the JR vector by incorporation of the E. coli beta galactosidase gene (PstI to SalI fragment of pBAG gag - beta gal fusion protein) into the BamH1 site of JR.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This cell line was derived from PA317 (see ATCC CRL-9098) by introduction of a retrovirus vector (JR-gal).
The vector is derived from the JR vector by incorporation of the E. coli beta galactosidase gene (PstI to SalI fragment of pBAG gag - beta gal fusion protein) into the BamH1 site of JR.
Comments
This cell line was derived from PA317 (see ATCC CRL-9098) by introduction of a retrovirus vector (JR-gal).
The vector is derived from the JR vector by incorporation of the E. coli beta galactosidase gene (PstI to SalI fragment of pBAG gag - beta gal fusion protein) into the BamH1 site of JR.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor
Wisconsin Alumni Res. Fndn.
Deposited As
Mus musculus
U.S. Patent Number
References

Gould MN, Wang B. Method for producing a recombinant mammal in vivo. US Patent 5,215,904 dated Jun 1 1993

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