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PFHR 9 [PFHR-9]
PFHR 9 [PFHR-9]
規(guī)格:
價格:詢價
編號:B165497
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
PFHR 9 [PFHR-9]
商品貨號
B165497
Organism
Mus musculus, mouse
Cell Type
Epithelial,Epithelial-like
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
embryonal carcinoma
Age
embryo
Applications
PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974.
The PFHR 9 cells were not tumorigenic when injected subcutaneously into 129/Sv mice whereas the parental line did produce tumors.
The cells produce basement membrane proteins - laminin-1; entactin-1; perlecan and collagen IV.
The cell line is useful for the production of basement membrane proteins and as a model to study the assembly and secretion of these proteins.
Storage Conditions
liquid nitrogen vapor phase
Derivation
PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974.
Genes Expressed
laminin-1; entactin-1; perlecan and collagen IV
Cellular Products
laminin-1; entactin-1; perlecan and collagen IV
Tumorigenic
No
Effects
No, not tumorigenic when injected subcutaneously into 129/Sv mice
Comments
PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974.
While PCC4F cells grow in suspension, the PRHR cell line is adherent. The cells are epithelial-like and have numerous vacuoles. PFHR 9 cells have a reported doubling time of 12 hours.
The PFHR 9 cells were not tumorigenic when injected subcutaneously into 129/Sv mice whereas the parental line did produce tumors.
The cells produce basement membrane proteins - laminin-1; entactin-1; perlecan and collagen IV.
The cell line is useful for the production of basement membrane proteins and as a model to study the assembly and secretion of these proteins.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Note: Do not let the cells overgrow.

Subcultivation Ratio: 1:5 to 1:7
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time
12 hrs
Name of Depositor
A Chung
Deposited As
mouse
References

Chung AE, et al. Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F. Cancer Res. 37: 2072-2081, 1977. PubMed: 558822

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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