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PLC/PRF/5
PLC/PRF/5
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165513
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
PLC/PRF/5
商品貨號(hào)
B165513
Organism
Homo sapiens, human
Tissue
liver
培養(yǎng)基
Cell Type
Alexander cells
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 [Cells contain hepatitis B]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hepatoma
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
ATCC CRL-8024 Cell Micrograph
Genes Expressed
hepatitis virus B surface antigen (HBsAg)
Cellular Products
hepatitis virus B surface antigen (HBsAg)
Comments
The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells secrete HBsAg.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 15,16
Name of Depositor
WJ McAleer
Deposited As
Homo sapiens
U.S. Patent Number
References

Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321

MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208

Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138

Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960

Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551

Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281

Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998

Cross References

Nucleotide (GenBank) : X75311 H.sapiens /Hepatitis B virus fusion mRNA for mevalonate kinase.

Nucleotide (GenBank) : X77700 H.sapiens / Hepatitis B virus (A4.0') DNA for mevalonate kinase (partial).

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