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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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當前位置: 首頁 > ATCC代理 > R2-10F6 [R2-10F6.3C10.3F9] B165558
R2-10F6 [R2-10F6.3C10.3F9]
R2-10F6 [R2-10F6.3C10.3F9]
規格:
價格:詢價
編號:B165558
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
R2-10F6 [R2-10F6.3C10.3F9]
商品貨號
B165558
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.
Derivation
The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
Clinical Data
R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40
Cellular Products
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40
Comments
The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.
R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Complete Growth Medium
Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2b
Name of Depositor
DH Presky
Year of Origin
1993
References

Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576

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