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當(dāng)前位置: 首頁 > ATCC代理 > R6.5.D6.E9.B2 [R6-5-D6] B165563
R6.5.D6.E9.B2 [R6-5-D6]
R6.5.D6.E9.B2 [R6-5-D6]
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165563
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
R6.5.D6.E9.B2 [R6-5-D6]
商品貨號(hào)
B165563
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
adult
Strain
BALB/c
Applications
The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)
Cellular Products
immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)
Comments
Animals were immunized with the EBV transformed lymphoblast cell line, JY.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG2a kappa
Population Doubling Time
12 hours
Name of Depositor
Dana Farber Cancer Inst.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Springer TA, et al. Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994

Mice were immunized with the EBV-transformed human lymphoblastoid cell line JY.

The antibody binds to ICAM-1(CD54), and inhibits the interaction of ICAM-1 with leukocytes via lymphocyte function antigen 1(LFA-1, CD11a/CD18).

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