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Ramos.2G6.4C10
Ramos.2G6.4C10
規格:
價格:詢價
編號:B165573
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Ramos.2G6.4C10
商品貨號
B165573
Organism
Homo sapiens, human
Cell Type
B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Burkitt's lymphoma (American)
Age
3 years
Gender
male
Ethnicity
Caucasian
Applications
Ramos.2G6.4C10 cells provide a sensitive and specific assay system for IL-4 that is insensitive to the presence of other lymphokines and cytokines such as interleukins (IL-2, IL-2 and IL-6), colony stimulating factors (G-CSF, M-CSF and GM-CSF) and interferons alpha and gamma (IFN alpha, IFN gamma). Tumor necrosis factors alpha and beta (TNF alpha, TNF beta) cause only minor interference.
The assay is sensitive to IL-4 concentrations in the range 50 to 800 pg/mL.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was derived from the Ramos (RA-1) cell line (see ATCC CRL-1596) by repetitive cloning with selection for low constitutive expression of CD23 and maximum stimulation of CD23 expression by IL-4.
Clinical Data
3 years
Caucasian
male
Antigen Expression
CD23 +
Receptor Expression
interleukin 4 (IL-4); low affinity IgE (CD23)
Genes Expressed
immunoglobulin (surface and secreted)
Cellular Products
immunoglobulin (surface and secreted)
Comments

Both the parental line (ATCC CRL-1596) and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.


Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 x 10 5 viable cells/mL and maintain between 1 and 2 x 10 6 cells/mL.
Medium Renewal: Add fresh medium every 4 to 6 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Isotype
IgM
Name of Depositor
JP Siegel
Deposited As
Homo sapiens
References

Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726

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