Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
Ewing's sarcoma
Age
19 years
Gender
male
Ethnicity
Caucasian
Derivation
The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus.
Clinical Data
19 years
Caucasian
male
Antigen Expression
Blood type B; Rh+
Comments
Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter.
The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
