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RL
RL
規格:
價格:詢價
編號:B165606
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
RL
商品貨號
B165606
Organism
Homo sapiens, human
Tissue
ascites
培養基
Cell Type
B lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
non-Hodgkin's lymphoma
Age
52 years
Gender
male
Ethnicity
Caucasian
Karyotype
the cells possess a 14:18 translocation
Derivation
RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Clinical Data
52 years
Caucasian
male
Antigen Expression
CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
Genes Expressed
immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Cellular Products
immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Comments
Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.

RL cells have been reported to be Epstein-Barr virus genome negative.


Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 and 1 x 106.


Medium Renewal: 2 to 3 times a week.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9,10
TPOX: 9,11
vWA: 19,20
Isotype
IgM; lambda light chain
Name of Depositor
M Beckwith
Deposited As
Homo sapiens
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

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