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RTG-2
RTG-2
規格:
價格:詢價
編號:B165637
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
RTG-2
商品貨號
B165637
Organism
Oncorhynchus mykiss, trout, rainbow
Tissue
mixed; testis; ovary
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender
male and female mixed
Storage Conditions
liquid nitrogen vapor phase
Virus Resistance
poliovirus 1
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with cold (less than 24°C) 0.25% (w/v) Trypsin - 0.03% EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.Cultures may be established with as few as 3 x 103 cells/mL.
  6. Incubate cultures at 22°C.

Medium Renewal: Once per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005.

Culture Conditions
Temperature: 22°C; cells do not survive above 26°C but will grow at temperatures as low as 4°C.
Name of Depositor
K Wolf
Deposited As
Salmo gairdneri
References

. . Science 135: 1065, 1962.

Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741

Cross References

Nucleotide (GenBank) : U82129 Oncorhynchus mykiss Y1 RNA, partial sequence.

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