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RTH-149
RTH-149
規(guī)格:
價格:詢價
編號:B165639
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
RTH-149
商品貨號
B165639
Organism
Oncorhynchus mykiss, trout, rainbow
Tissue
liver
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hepatoma
Storage Conditions
liquid nitrogen vapor temperature
Comments
Culture the cells at 21C.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature until the cells detach (about 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 21.0°C
Name of Depositor
JL Fryer
Deposited As
Salmo gairdneri
References

Lannan CN, et al. Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20: 671-676, 1984. PubMed: 6542066

Fryer J, et al. A cell line derived from rainbow trout (Salmo gairdneri) hepatoma. Fish Pathol 15:193-200, 1981.

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