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SC-1
SC-1
規格:
價格:詢價
編號:B165668
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SC-1
商品貨號
B165668
Organism
Homo sapiens, human
Cell Type
B lymphocyte; Epstein-Barr virus (EBV) transforme
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
The line is homozygous for the sickle cell allele at the hemoglobin beta chain locus.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp5 and 1.5 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 92.5%; DMSO, 7.5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,7.3
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8,10
THO1: 7
TPOX: 6,9
vWA: 14
Name of Depositor
Cetus Corp.
Deposited As
Homo sapiens
U.S. Patent Number
References

Mullis KB, et al. Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US Patent 4,683,195 dated Jul 28 1987

Mullis KB. Process for amplifying nucleic acid sequences. US Patent 4,683,202 dated Jul 28 1987

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