91极品国产,奇米成人影视,五月激情视频

SK-MES-1

規(guī)格:

價格:詢價

編號:B165712

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

SK-MES-1

商品貨號

B165712

Organism

Homo sapiens, human

Tissue

lung; Derived from Metastatic Site: pleural effusion

培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

squamous cell carcinoma

Age

65 years adult

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining.

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Clinical Data

65 years adult

Caucasian

male

Antigen Expression

Blood Type O; Rh+; HLA A3, Aw30, B7, B27

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO₂ in air atmosphere. ATCC tested fetal bovine serum is available as ATCC? Catalog No. 30-2020 (500 mL) and ATCC? Catalog No. 30-2021 (100 mL).

Subculturing

Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 12

D13S317: 11

D16S539: 13

D5S818: 11

D7S820: 8

THO1: 6,9.3

TPOX: 8

vWA: 14

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1-2

PGM1, 1-2

PGM3, 1

Name of Depositor

G Trempe, LJ Old

Deposited As

Homo sapiens

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

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