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SNU-C2A
SNU-C2A
規格:
價格:詢價
編號:B165743
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SNU-C2A
商品貨號
B165743
Organism
Homo sapiens, human
Product Format
frozen
培養基
Morphology
epithelial
Culture Properties
loosely adherent, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
colorectal carcinoma
Age
43 years
Gender
female
Ethnicity
Asian
Applications
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 46; range = 44 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations.
Images
Derivation
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
Clinical Data
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
female
Asian
43 years
Antigen Expression
Blood type A; Rh+
Genes Expressed
carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen,Blood type A; Rh+
Cellular Products
carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum).
The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.
Complete Growth Medium
ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  1. 0.02 mg/ml insulin
  2. 0.01 mg/ml transferrin
  3. 25 nM sodium selenite
  4. 50 nM Hydrocortisone
  5. 1 ng/ml Epidermal Growth Factor (do not filter)
  6. 0.01 mM ethanolamine
  7. 0.01 mM phosphorylethanolamine
  8. 100 pM triiodothyronine
  9. 0.5% (w/v) bovine serum albumin
  10. 10 mM HEPES
  11. 0.5 mM sodium pyruvate
  12. 2mM L-glutamine(for final conc. of 4.5 mM)
  13. 10% FBS

Subculturing
Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.
Medium Renewal: Two times weekly
Note: Growth is slow.
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additonal 10% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11, 14
D13S317: 9, 12
D16S539: 8, 9
D5S818: 8, 9
D7S820: 13, 16
THO1: 9
TPOX: 8
vWA: 15, 19, 20

Species confirmation via CO1 assay: Human (mouse material may also be detected, likely due to this cell line being a nude mouse xenograft of tumor fragments from a human patient)
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor
AF Gazdar
Deposited As
Homo sapiens
Passage History
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
Year of Origin
1982
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

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