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SR-4987
SR-4987
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B165752
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SR-4987
商品貨號(hào)
B165752
Organism
Mus musculus, mouse
Tissue
bone marrow
培養(yǎng)基
Cell Type
virus transformed
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Age
5 months
Gender
female
Strain
BDF1
Applications
transfection host
Storage Conditions
liquid nitrogen vapor phase
Karyotype
tetraploid
Derivation
The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
Clinical Data
female
Genes Expressed
macrophage colony stimulating factor (MCSF)
Cellular Products
macrophage colony stimulating factor (MCSF)
Tumorigenic
Yes
Effects
Yes, produce sarcomas in syngeneic mice
Comments
The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
The cells produce MCSF, and are highly sensitive to fibroblast growth factor (FGF).
They are poorly clonogenic in soft agar.
Neither interleukin 3 (IL-3) or granulocyte colony stimulating factor (GCSF) were detected in SR-4987 conditioned medium.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:4 to 1:8
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time
15 hrs
Name of Depositor
G Zaleskis
Deposited As
Mus musculus
References

Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506

Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333

Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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