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SU.86.86
SU.86.86
規格:
價格:詢價
編號:B165759
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SU.86.86
商品貨號
B165759
Organism
Homo sapiens, human
Tissue
pancreas; derived from metastatic site: liver
培養基
Product Format
frozen
Morphology
Epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
ductal carcinoma
Age
57 years
Gender
Female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Images
CRL-1837 Micrograph
Derivation

This cell line was developed from a wedge biopsy of a liver metastasis of a pancreatic ductal carcinoma.

Clinical Data
57 years
Caucasian
female
Genes Expressed
carcinoembryonic antigen (CEA)
Tumorigenic
Yes
Effects
Yes, in nude mice inoculated subcutaneously with 10(7) cells.
Comments

The cells can be lysed completely in vitro by autologous and allogeneic lymphokine activated killer (LAK) cells in the presence of interleukin 2 (IL-2). They are relatively insensitive to autologous and allogeneic natural killer (NK) cell lysis.

The cells produce CEA, and grow in orthotopic and heterotopic positions in nude mice within 21 days at 100% frequency (5/5).

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium. 
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and place at 37°C to facilitate dispersal. Observe cells within 5 to 10 minutes under an inverted microscope 
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. 
  6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Recommended seeding densities of 5 x 103 to 4 x 104 viable cells/cm2 
  7. Place culture vessels in incubators at 37°C. Note: subcultures can also be prepared by scraping the cells from the floor of the flask. Add fresh medium, aspirate the scraped cells to disperse them and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended by depositor
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Culture medium ,40% ; DMSO,10% ; FBS, 50% FBS..
Storage temperature: liquid nitrogen vapor phase
STR Profile

D5S818: 7,13
D13S317: 11,12
D7S820: 8,11
D16S539: 11,13
vWA: 17
THO1: 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 10,11
Name of Depositor
WD Holder
Deposited As
Homo sapiens
References

Drucker BJ, et al. A new human pancreatic carcinoma cell line produced for adoptive immunotherapy studies with lymphokine-activated killer cells in nude mice. In Vitro Cell. Dev. Biol. 24: 1179-1187, 1988. PubMed: 3264833

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