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Swiss SFME (Serum Free Mouse Embryo)

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價格:詢價

編號:B165804

品牌:Mingzhoubio

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定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Swiss SFME (Serum Free Mouse Embryo)

商品貨號

B165804

Organism

Mus musculus, mouse

Tissue

embryo

培養(yǎng)基

Cell Type

Astrocyte

Product Format

frozen

Morphology

fibroblast

Culture Properties

clusters in suspension; the cells will attach to flasks coated with 0.02 mg/ml human fibronectin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

embryo; 16 days

Strain

Swiss

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Karyotype

modal number = 40; range = 39 to 42

Derivation

This line was derived from minced, trypsinized Swiss mouse embryos grown in serum free medium.

Tumorigenic

Effects

No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Comments

Either serum or TGF-beta induce astrocyte differentiation accompanied by GFAP (glial fibrillary acidic protein) expression. Furthermore, the presence of serum causes cell growth arrest.

This process is reversible upon removal of the serum.

Swiss SFME cells do not grow in conventional media supplemented with fetal bovine serum or bovine calf serum.

When cultured in the serum free medium (see below), Swiss SFME cells reportedly can be propagated for extended periods without undergoing"crisis" or gross chromosomal aberration.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50%; Ham's F12 medium, 50%, Supplements: 0.01 mg/ml bovine insulin, 0.025 mg/ml human transferrin, 0.02 mg/ml human high density lipoprotein (HDL), 100 ng/ml mouse epidermal growth factor (EGF), 10 nM sodium selenite, 1 unit/ml human platelet derived growth factor (PDGF), and 15 mM HEPES. NOTE: HDL is available from Sigma Chemical Co. (catalog number L2014).

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:100 is recommended

Medium Renewal: Twice per week

Remove medium, and collect suspended clusters by centrifugation.

To disperse clusters or to remove adherent cells, add fresh 0.25% Trypsin, 0.02% EDTA at room temperature until the cells disperse (2 to 3 minutes).

Add an equal volume of fresh serum free medium plus 0.1% soybean trypsin inhibitor and centrifuge at 1000 X g.

Resuspend the cell pellet in growth medium, aspirate and dispense into new (fibronectin coated, if adherence is desired) flasks.

Floating clusters may also be remove using a pipette and transferred to a flask containing fresh growth medium.

Cryopreservation

culture medium, 80%; fetal bovine serum, 10%; DMSO, 10%

Name of Depositor

Oregon State University

U.S. Patent Number

5,474,930

References

Loo DT, et al. Extended culture of mouse embryo cells without senescence: inhibition by serum. Science 236: 200-202, 1987. PubMed: 3494308

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