黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > TCCSUP B165830
TCCSUP
TCCSUP
規格:
價格:詢價
編號:B165830
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TCCSUP
商品貨號
B165830
Organism
Homo sapiens, human
Tissue
urinary bladder
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
grade IV transitional cell carcinoma
Age
67 years
Gender
female
Storage Conditions
liquid nitrogen vapor phase
Karyotype
(P12 and 35) hypotetraploid with marker chromosomes
Derivation
The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder.
Clinical Data
female

Antigen Expression
HLA 2, 3, 7, 12
Comments

The patient had a 4 month history of hematuria prior to removal of the tumor.

Metastases to the bone marrow were discovered later.

Studies on ultrastructure indicated presence of microvilli and lipid bodies but no desmosomes were observed.
Complete Growth Medium
Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids (ATCC 30-2003) and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended 

Medium Renewal: 2 to 3 times per week Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9,11
D5S818: 12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 14,16
Isoenzymes
AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 1
Name of Depositor
C O'Toole
References

O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756

  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479
  • 曹經理
    手機號:18662422060
    QQ:3671869992