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TE4
TE4
規(guī)格:
價格:詢價
編號:B165846
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
TE4
商品貨號
B165846
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an intracellular antigen expressed in the endocrine thymic epithelium.
TE4+ cells strongly express class I and class II histocompatibility antigens, and are positive for thymosin alpha-1 and keratin.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human thymus (thymic endocrine epithelium)
Cellular Products
immunoglobulin; monoclonal antibody; against human thymus (thymic endocrine epithelium)
Comments
Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with an intracellular antigen expressed in the endocrine thymic epithelium.
It defines the mesodermal portion of the thymic microenvironment that may participate in conferring MHC restriction to maturing T lymphocytes.
TE4+ cells strongly express class I and class II histocompatibility antigens, and are positive for thymosin alpha-1 and keratin.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
heat-inactivated fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
BF Haynes
Deposited As
mouse (B cell); mouse (myeloma)
References

Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562

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