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TF-1.CN5a.1
TF-1.CN5a.1
規(guī)格:
貨期:
編號(hào):B165852
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
TF-1.CN5a.1
商品貨號(hào)
B165852
Organism
Homo sapiens, human
Tissue
bone marrow
Cell Type
erythroblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells contain CMV viral sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
erythroleukemia
Age
35 years
Gender
male
Ethnicity
Japanese
Applications
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
The cells can be used to access the potency and to study signal transduction of CNTF.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
Clinical Data
male
Japanese
35 years
Receptor Expression
alpha subunit of human ciliary neurotrophic factor (CNTF) (receptor is not expressed by TF-1 cells)
Comments
The cells stably express the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor and CNTF supports the short term proliferation of the cells. Suggested assay conditions are washing the cells with RPMI to remove GM-CSF, then incubation in RPMI 1640 95%, fetal bovine serum 5%, and picogram/ml concentrations of CNTF. The cells maintain responsiveness for at least 193 population doublings in full maintenance medium.
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
TF-1 cells were transfected, using Transfectam, with the gene for the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor ligated to the pCR3.1 vector.
The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene.
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
TF-1.CN5a cells were grown, transiently, in the absence of GM-CSF and with 2 ng/ml CNTF to establish the TF-1.CN5a.1 cell line.
The cells can be used to access the potency and to study signal transduction of CNTF.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 2 ng/ml GM-CSF and 0.4 mg/ml G-418, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cell density at a cell concentration between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal:  Every 2 to 3 days

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Name of Depositor
CJ Robinson
Deposited As
human
Year of Origin
2003
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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