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Toledo
Toledo
規格:
價格:詢價
編號:B165874
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Toledo
商品貨號
B165874
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Age
adult
Gender
female
Ethnicity
White
Applications
This cell line is a model system for studying non-Hodgkin lymphomas.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL).
Clinical Data
adult
female
White
Antigen Expression
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 -
Comments
Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain.
Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations.
The cells do not express surface or cytoplasmic immunoglobulin.
The cells are negative for Epstein-Barr virus.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
D5S818:10,12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,11
vWA: 16,17
Amelogenin: X
CSF1PO: 12
D13S317:11
D16S539:9
Population Doubling Time
24 to 30 hrs
Name of Depositor
H Ben-Bassat
Deposited As
human
Year of Origin
1990
References

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

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